|
KMID : 0383820110700050390
|
|
Tuberculosis and Respiratory Diseases
2011 Volume.70 No. 5 p.390 ~ p.396
|
|
|
The Effect of Hyaluronan Treatment in Endotoxemic Rats
|
|
Rho Byung-Hak
Kwon Kun-Young
Choi Won-Il
|
|
|
Abstract
|
|
|
Background: Hyaluronan (HA) is an unbranched glycosaminoglycan. It has been proposed that HA acts as a vehicle for cytokines due to the strong negative charge on its surface. We hypothesized that HA would function like a cytokine scavenger and reduce the inflammatory signaling cascade and this would lead to improved survival in rats suffering with endotoxemia.
Methods: Endotoxin (Salmonella, 10 mg/kg) or an equal amount of 0.9% NaCl (NS) was injected into the jugular vein of rats. HA (1,600 kDa, 0.35%) or NS was given at 0.1 mL/kg/h for 3 hours. HA or NS infusion was started at 4 hour after endotoxin injection. The rats were divided into the control and HA groups (n=16 for each group). The mean arterial pressure (MAP) was monitored during HA or normal saline infusion. Survival was assessed every 12 hours for 3 days throughout the experiment.
Results: The survival rate (%) of the rats treated with HA was higher (60%) than that of the controls (20%) when HA was infused 4 hours after lipopolysaccharide (LPS) injection. The bronchoalveolar lavage (BAL) fluid of the animals surviving HA or NS infusion 4 hours after LPS showed that the total cell counts and number of neutrophils were significantly (p＜0.01) reduced in the HA treated groups compared with that of the controls (total cell count, 9.2×104/mL vs. 61×104/mL; neutrophils, 21×104/mL vs. 0.2×104/mL, respectively). There was no significant MAP difference between the HA or control groups either with or without endotoxin.
Conclusion: Infusion of hyaluronan (1,600 kDa) reduced the BAL total cell count and the number of neutrophils and it improved the survival rate of the endotoxemic rats.
|
|
|
KEYWORD
|
|
|
Endotoxemia, Hyaluronic Acid, Survival
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|